This online tool assists molecular biologists in determining the optimal conditions for DNA ligation reactions, typically using enzymes supplied by New England Biolabs (NEB). It provides recommendations for enzyme and cofactor concentrations, incubation times, and temperatures based on the specific DNA fragments being joined, including factors like fragment size and type (blunt or cohesive ends).
Accurate ligation is fundamental to many molecular biology procedures such as cloning, subcloning, and DNA library construction. A dedicated calculation tool streamlines experimental design by simplifying complex calculations and minimizing the need for trial-and-error optimization. This improves the efficiency and reliability of experiments, contributing to the rapid advancement of scientific discovery.
